ABSTRACT
A reverse phase high performance liquid chromatography method was developed for the determination of diloxanide furoate and metronidazole in tablets. The separation was achieved using C8 column [4.6 mm x 250 mm] 5 micro m and mobile phase acetonitrite: water [65:35] v/v, at a flow rate of 1ml/min. Detection was carried out using UV at 230 nm. The retention times of metronidazole and diloxanide furoate were 3.17 and 5.18 min. the detection limits for metronidazole and diloxanide furoate were 0.03, 0.04 micro g/ml and the Quantitation limits were 0.11, 0.13 micro g/ml respectively. The method has validated for accuracy, precision, selectivity and robustness, Linearity was in the range of 32 - 48 micro g/ml for metronidazole, and 40 -60 micro g/ml for diloxanide furoate. The recovery values of Accuracy were 99.08 - 100.52% for metronidazole and diloxanide furoate respectively